› 6lyd

X-ray diffraction
2.6Å resolution

Crystal Structure of mimivirus UNG Y322L in complex with UGI

Released:
DOI: 10.2210/pdb6lyd/pdb
PDB entry 6lyd coloured by chain, front view.
PDB entry 6lyd coloured by chain, side view.
PDB entry 6lyd coloured by chain, top view.
Source organisms:
Primary publication:
Selective interactions between mimivirus uracil-DNA glycosylase and inhibitory proteins determined by a single amino acid.
Pathak D, Kwon E, Kim DY
J Struct Biol 211 107552 (2020)
PMID: 32569642

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147056 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Probable uracil-DNA glycosylase Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.8 KDa
Source organism: Acanthamoeba polyphaga mimivirus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5UPT2 (Residues: 95-370; Coverage: 75%)
Gene names: MIMI_L249, UNG
Sequence domains: Uracil DNA glycosylase superfamily
Uracil-DNA glycosylase inhibitor Chain: I
Molecule details ›
Chain: I
Length: 83 amino acids
Theoretical weight: 9.35 KDa
Source organism: Bacillus phage PBS2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14739 (Residues: 2-84; Coverage: 99%)
Gene name: UGI
Sequence domains: Uracil-DNA glycosylase inhibitor

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 11C
Spacegroup: P61
Unit cell:
a: 103.766Å b: 103.766Å c: 81.523Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.17 0.227
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Viruses with U-DNA: New Avenues for Biotechnology.
Nagy et al. (2021)