› 6m0w

X-ray diffraction
2.76Å resolution

Crystal structure of Streptococcus thermophilus Cas9 in complex with the AGAA PAM

Released:
DOI: 10.2210/pdb6m0w/pdb
PDB entry 6m0w coloured by chain, front view.
PDB entry 6m0w coloured by chain, side view.
PDB entry 6m0w coloured by chain, top view.
Source organisms:
Primary publication:
Catalytic-state structure and engineering of Streptococcus thermophilus Cas9
Zhang Y, Zhang H, Xu X, Wang Y, Chen W, Wang Y, Wu Z, Tang N, Wang Y, Zhao S, Gan J, Ji Q
Nat Catal (2020)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120254 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
CRISPR-associated endonuclease Cas9 1 Chain: A
Molecule details ›
Chain: A
Length: 1122 amino acids
Theoretical weight: 129.65 KDa
Source organism: Streptococcus thermophilus LMD-9
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03LF7 (Residues: 2-1121; Coverage: 100%)
Gene names: STER_0709, cas9-1, csn1
Sequence domains:
DNA (28-MER) Chain: C
Molecule details ›
Chain: C
Length: 28 nucleotides
Theoretical weight: 8.5 KDa
Source organism: Streptococcus thermophilus
Expression system: Not provided
DNA (5'-D(*AP*AP*AP*GP*AP*AP*GP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 8 nucleotides
Theoretical weight: 2.47 KDa
Source organism: Streptococcus thermophilus
Expression system: Not provided
RNA (72-MER) Chain: B
Molecule details ›
Chain: B
Length: 72 nucleotides
Theoretical weight: 23.23 KDa
Source organism: Streptococcus thermophilus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 5 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: C2
Unit cell:
a: 321.708Å b: 75.333Å c: 70.3Å
α: 90° β: 93.47° γ: 90°
R-values:
R R work R free
0.222 0.22 0.26
Expression systems:
  • Escherichia coli
  • Not provided

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