› 6m37

X-ray diffraction
3.1Å resolution

The crystal structure of B. subtilis RsbV/RsbW complex in the hexagonal crystal form

Released:
DOI: 10.2210/pdb6m37/pdb
PDB entry 6m37 coloured by chain, front view.
PDB entry 6m37 coloured by chain, side view.
PDB entry 6m37 coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Structural insights into the regulation of SigB activity by RsbV and RsbW.
Pathak D, Jin KS, Tandukar S, Kim JH, Kwon E, Kim DY
IUCrJ 7 737-747 (2020)

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148211 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine-protein kinase RsbW Chains: A, C
Molecule details ›
Chains: A, C
Length: 141 amino acids
Theoretical weight: 15.7 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: P17904 (Residues: 5-145; Coverage: 88%)
Gene names: BSU04720, rsbW
Sequence domains: Histidine kinase-like ATPase domain
Anti-sigma-B factor antagonist Chains: B, D
Molecule details ›
Chains: B, D
Length: 103 amino acids
Theoretical weight: 11.35 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: P17903 (Residues: 2-104; Coverage: 95%)
Gene names: BSU04710, rsbV
Sequence domains: STAS domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 11C
Spacegroup: P6422
Unit cell:
a: 158.2Å b: 158.2Å c: 96.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.222 0.252
Expression system: Escherichia coli

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