› 6m48

X-ray diffraction
2.5Å resolution

Crystal structure of pilus adhesin, SpaC from Lactobacillus rhamnosus GG - P21212 form

Released:
DOI: 10.2210/pdb6m48/pdb
PDB entry 6m48 coloured by chain, front view.
PDB entry 6m48 coloured by chain, side view.
PDB entry 6m48 coloured by chain, top view.
Source organism: Lacticaseibacillus rhamnosus GG
Primary publication:
Crystal structure of lactobacillar SpaC reveals an atypical five-domain pilus tip adhesin: Exposing its substrate-binding and assembly in SpaCBA pili.
Kant A, Palva A, von Ossowski I, Krishnan V
J Struct Biol 211 107571 (2020)
PMID: 32653644

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167049 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SpaC Chains: A, B
Molecule details ›
Chains: A, B
Length: 836 amino acids
Theoretical weight: 90.94 KDa
Source organism: Lacticaseibacillus rhamnosus GG
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21212
Unit cell:
a: 116.181Å b: 128.128Å c: 136.358Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.235
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Probiotic Gastrointestinal Transit and Colonization After Oral Administration: A Long Journey.
Han et al. (2021)
3 other articles

2 mentions without citation

Assessment of domain interactions in the fourteenth round of the Critical Assessment of Structure Prediction (CASP14).
Schaeffer et al. (2021)
1 more