› 6m4e

X-ray diffraction
2.1Å resolution

Crystal structure of a GH1 beta-glucosidase from Hamamotoa singularis

Released:
DOI: 10.2210/pdb6m4e/pdb
Model geometry
Fit model/data
PDB entry 6m4e coloured by chain, front view.
PDB entry 6m4e coloured by chain, side view.
PDB entry 6m4e coloured by chain, top view.
Source organism: Hamamotoa singularis
Primary publication:
Crystal structure of a GH1 β-glucosidase from Hamamotoa singularis.
Uehara R, Iwamoto R, Aoki S, Yoshizawa T, Takano K, Matsumura H, Tanaka SI
Protein Sci 29 2000-2008 (2020)
PMID: 32713015

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176374 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Beta-galactosidase-like enzyme Chain: A
Molecule details ›
Chain: A
Length: 578 amino acids
Theoretical weight: 64.35 KDa
Source organism: Hamamotoa singularis
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q564N5 (Residues: 23-594; Coverage: 99%)
Gene name: bglA
Sequence domains: Glycosyl hydrolase family 1

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand MAN 3 x MAN
Ligand NAG 1 x NAG
Ligand EDO 53 x EDO
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: C222
Unit cell:
a: 160.79Å b: 172.83Å c: 67.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.174 0.197
Expression system: Komagataella pastoris

Quick links

Citations

1 review citation

Fungal cellulases: protein engineering and post-translational modifications.
Zhang et al. (2022)
2 other articles

2 mentions without citation

Crystal structure of a GH1 β-glucosidase from Hamamotoa singularis.
Uehara et al. (2020)
1 more