› 6m4j

X-ray diffraction
1.8Å resolution

SspA in complex with cysteine

Released:
DOI: 10.2210/pdb6m4j/pdb
PDB entry 6m4j coloured by chain, front view.
PDB entry 6m4j coloured by chain, side view.
PDB entry 6m4j coloured by chain, top view.
Source organism: Vibrio cyclitrophicus
Primary publication:
Structural Analysis of an l-Cysteine Desulfurase from an Ssp DNA Phosphorothioation System.
Liu L, Jiang S, Xing M, Chen C, Lai C, Li N, Liu G, Wu D, Gao H, Hong L, Tan P, Chen S, Deng Z, Wu G, Wang L
mBio 11 (2020)
PMID: 32345643

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167050 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SspA complex protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 348 amino acids
Theoretical weight: 37.9 KDa
Source organism: Vibrio cyclitrophicus
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand PLP 2 x PLP
Ligand CYS 2 x CYS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P6322
Unit cell:
a: 132.264Å b: 132.264Å c: 152.636Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.167 0.166 0.19
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The Multifaceted Bacterial Cysteine Desulfurases: From Metabolism to Pathogenesis.
Das et al. (2021)
1 more

1 mention without citation

Structural Analysis of an l-Cysteine Desulfurase from an Ssp DNA Phosphorothioation System.
Liu et al. (2020)