› 6m7z

X-ray diffraction
2.5Å resolution

A divergent kinase lacking the glycine-rich loop regulates membrane ultrastructure of the Toxoplasma parasitophorous vacuole

Released:
DOI: 10.2210/pdb6m7z/pdb
Model geometry
Fit model/data
PDB entry 6m7z coloured by chain, front view.
PDB entry 6m7z coloured by chain, side view.
PDB entry 6m7z coloured by chain, top view.
Source organism: Toxoplasma gondii RH
Primary publication:
Divergent kinase regulates membrane ultrastructure of the Toxoplasma parasitophorous vacuole.
Beraki T, Hu X, Broncel M, Young JC, O'Shaughnessy WJ, Borek D, Treeck M, Reese ML
OpenAccess logo Proc Natl Acad Sci U S A 116 6361-6370 (2019)
PMID: 30850550

Function and Biology Details

Reaction catalysed: 
Triphosphate + [microsomal-membrane protein] = diphosphate + [microsomal-membrane protein] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105866 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bradyzoite pseudokinase 1 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 319 amino acids
Theoretical weight: 35.94 KDa
Source organism: Toxoplasma gondii RH
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A7J6KD88 (Residues: 60-377; Coverage: 91%)
Gene names: BPK1, TGRH88_004990

Ligands and Environments

2 bound ligands:
Ligand EDO 16 x EDO
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 171.466Å b: 123.068Å c: 86.615Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.239
Expression system: Escherichia coli BL21

Quick links

Citations

6 review citations

There's more to death than life: Noncatalytic functions in kinase and pseudokinase signaling.
Mace et al. (2021)
5 more

2 mentions without citation

Divergent kinase regulates membrane ultrastructure of the Toxoplasma parasitophorous vacuole.
Beraki et al. (2019)
1 more