› 6m84

X-ray diffraction
2.81Å resolution

Crystal structure of cKir2.2 force open mutant in complex with PI(4,5)P2

Released:
DOI: 10.2210/pdb6m84/pdb
PDB entry 6m84 coloured by chain, front view.
PDB entry 6m84 coloured by chain, side view.
PDB entry 6m84 coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Atomistic basis of opening and conduction in mammalian inward rectifier potassium (Kir2.2) channels.
Zangerl-Plessl EM, Lee SJ, Maksaev G, Bernsteiner H, Ren F, Yuan P, Stary-Weinzinger A, Nichols CG
J Gen Physiol 152 (2020)
PMID: 31744859

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123320 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-sensitive inward rectifier potassium channel 12 Chain: A
Molecule details ›
Chain: A
Length: 343 amino acids
Theoretical weight: 39.48 KDa
Source organism: Gallus gallus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: F1NHE9 (Residues: 38-369; Coverage: 77%)
Gene name: KCNJ12
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand K 6 x K
Ligand PIO 1 x PIO
Ligand LMT 1 x LMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: I4
Unit cell:
a: 82.787Å b: 82.787Å c: 182.976Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.222 0.269
Expression system: Komagataella pastoris

Quick links

Citations

1 review citation

From Crosstalk to Synergism: The Combined Effect of Cholesterol and PI(4,5)P2 on Inwardly Rectifying Potassium Channels.
Bukiya et al. (2023)
11 other articles

7 mentions without citation

Atomistic basis of opening and conduction in mammalian inward rectifier potassium (Kir2.2) channels.
Zangerl-Plessl et al. (2020)
6 more