› 6m8s

X-ray diffraction
3.71Å resolution

Crystal structure of the KCTD12 H1 domain in complex with Gbeta1gamma2 subunits

Released:
DOI: 10.2210/pdb6m8s/pdb
PDB entry 6m8s coloured by chain, front view.
PDB entry 6m8s coloured by chain, side view.
PDB entry 6m8s coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for KCTD-mediated rapid desensitization of GABAB signalling.
Zheng S, Abreu N, Levitz J, Kruse AC
Nature 567 127-131 (2019)
PMID: 30814734

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
PDBe Complex ID:
PDB-CPX-157921 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chains: C, D, G, H, K
Molecule details ›
Chains: C, D, G, H, K
Length: 350 amino acids
Theoretical weight: 38.53 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chains: E, F, I, J, L
Molecule details ›
Chains: E, F, I, J, L
Length: 71 amino acids
Theoretical weight: 7.85 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Structure domains: Transducin (heterotrimeric G protein), gamma chain
BTB/POZ domain-containing protein KCTD12 Chains: A, B, M, O, P
Molecule details ›
Chains: A, B, M, O, P
Length: 129 amino acids
Theoretical weight: 14.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q96CX2 (Residues: 200-325; Coverage: 39%)
Gene names: C13orf2, KCTD12, KIAA1778, PFET1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 109.09Å b: 121.99Å c: 206.43Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.253 0.251 0.287
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

13 review citations

KCTD: A new gene family involved in neurodevelopmental and neuropsychiatric disorders.
Teng et al. (2019)
12 more

3 mentions without citation

Structural basis for KCTD-mediated rapid desensitization of GABAB signalling.
Zheng et al. (2019)
2 more