› 6mc9

X-ray diffraction
3.3Å resolution

Crystal Structure of Human Nav1.4 C-Terminal (1599-1754) domain in complex with calcium-bound calmodulin

Released:
DOI: 10.2210/pdb6mc9/pdb
PDB entry 6mc9 coloured by chain, front view.
PDB entry 6mc9 coloured by chain, side view.
PDB entry 6mc9 coloured by chain, top view.
Source organisms:
Primary publication:
Ca2+-dependent regulation of sodium channels NaV1.4 and NaV1.5 is controlled by the post-IQ motif.
Yoder JB, Ben-Johny M, Farinelli F, Srinivasan L, Shoemaker SR, Tomaselli GF, Gabelli SB, Amzel LM
Nat Commun 10 1514 (2019)
PMID: 30944319

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-144029 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Sodium channel protein type 4 subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 168 amino acids
Theoretical weight: 19.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P35499 (Residues: 1599-1754; Coverage: 9%)
Gene name: SCN4A
Calmodulin-1 Chain: B
Molecule details ›
Chain: B
Length: 149 amino acids
Theoretical weight: 16.85 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0DP29 (Residues: 1-149; Coverage: 100%)
Gene names: CaMI, Calm, Calm1, Cam, Cam1
Sequence domains: EF-hand domain pair

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-2
Spacegroup: P43212
Unit cell:
a: 72.615Å b: 72.615Å c: 134.661Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.24 0.285
Expression system: Escherichia coli BL21

Quick links

Citations

6 review citations

Structure and function of STAC proteins: Calcium channel modulators and critical components of muscle excitation-contraction coupling.
Rufenach et al. (2021)
5 more

2 mentions without citation

Structural basis of cytoplasmic NaV1.5 and NaV1.4 regulation.
Nathan et al. (2021)
1 more