› 6mf0

X-ray diffraction
3.2Å resolution

Crystal Structure Determination of Human/Porcine Chimera Coagulation Factor VIII

Released:
DOI: 10.2210/pdb6mf0/pdb
PDB entry 6mf0 coloured by chain, front view.
PDB entry 6mf0 coloured by chain, side view.
PDB entry 6mf0 coloured by chain, top view.
Source organisms:
Primary publication:
The 3.2 Å structure of a bioengineered variant of blood coagulation factor VIII indicates two conformations of the C2 domain.
Smith IW, d'Aquino AE, Coyle CW, Fedanov A, Parker ET, Denning G, Spencer HT, Lollar P, Doering CB, Spiegel PC
J Thromb Haemost 18 57-69 (2020)
PMID: 31454152

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-132517 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (5 distinct):
Coagulation factor VIII Chains: A, B
Molecule details ›
Chains: A, B
Length: 1467 amino acids
Theoretical weight: 168.29 KDa
Source organisms: Expression system: Cricetulus griseus
UniProt:
  • Canonical: P12263 (Residues: 1-405, 762-1820; Coverage: 37%)
  • Canonical: P00451 (Residues: 406-761, 2039-2351; Coverage: 29%)
Gene names: CF8, F8, F8C
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
3 bound ligands:
Ligand CA 2 x CA
Ligand ZN 2 x ZN
Ligand CU1 2 x CU1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: P21
Unit cell:
a: 72.005Å b: 135.863Å c: 196.107Å
α: 90° β: 90.151° γ: 90°
R-values:
R R work R free
0.21 0.206 0.287
Expression system: Cricetulus griseus

Quick links

Citations

3 review citations

Factor VIII and Factor V Membrane Bound Complexes.
Stoilova-McPhie S. (2021)
2 more

4 mentions without citation

The 3.2 Å structure of a bioengineered variant of blood coagulation factor VIII indicates two conformations of the C2 domain.
Smith et al. (2020)
3 more