6mfr

X-ray diffraction
3.6Å resolution

Human Argonaute2-miR-122 bound to a target RNA with three central mismatches (bu3)

Released:
Model geometry
Data not analysed
Fit model/data
Data not analysed

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-193888 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Protein argonaute-2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 859 amino acids
Theoretical weight: 97.36 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9UKV8 (Residues: 1-859; Coverage: 100%)
Gene names: AGO2, EIF2C2
Sequence domains:
RNA (5'-R(P*UP*GP*GP*AP*GP*UP*GP*UP*GP*AP*CP*AP*AP*UP*GP*GP*UP*GP*UP*U)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 21 nucleotides
Theoretical weight: 6.79 KDa
Source organism: Homo sapiens
Expression system: Not provided
RNA (5'-R(P*AP*AP*AP*CP*AP*CP*CP*AP*UP*UP*UP*UP*CP*AP*CP*AP*CP*UP*CP*CP*AP*AP*A)-3') Chains: E, F

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 109.174Å b: 137.96Å c: 153.122Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.239 0.283
Expression systems:
  • Spodoptera frugiperda
  • Not provided