› 6mgl

X-ray diffraction
1.5Å resolution

Crystal structure of the catalytic domain from GH74 enzyme PoGH74 from Paenibacillus odorifer, D60A mutant in complex with XXLG and XGXXLG xyloglucan

Released:
DOI: 10.2210/pdb6mgl/pdb
PDB entry 6mgl coloured by chain, front view.
PDB entry 6mgl coloured by chain, side view.
PDB entry 6mgl coloured by chain, top view.
Source organism: Paenibacillus odorifer
Primary publication:
Structural enzymology reveals the molecular basis of substrate regiospecificity and processivity of an exemplar bacterial glycoside hydrolase family 74 endo-xyloglucanase.
Arnal G, Stogios PJ, Asohan J, Skarina T, Savchenko A, Brumer H
Biochem J 475 3963-3978 (2018)
PMID: 30463871

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103664 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
CBM3 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 747 amino acids
Theoretical weight: 80.14 KDa
Source organism: Paenibacillus odorifer
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1R0YRH0 (Residues: 35-779; Coverage: 75%)
Gene name: BSK60_29080
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, XYS, GAL
Carbohydrate polymer
Carbohydrate polymer : NEW Components: BGC, XYS, GAL
Carbohydrate polymer
4 bound ligands:
Ligand CL 2 x CL
Ligand MG 3 x MG
Ligand PE3 1 x PE3
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 61.17Å b: 100.84Å c: 118.689Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 0.148 0.177
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Cell Surface Xyloglucan Recognition and Hydrolysis by the Human Gut Commensal Bacteroides uniformis.
Grondin et al. (2022)
1 more

2 mentions without citation

Substrate specificity, regiospecificity, and processivity in glycoside hydrolase family 74.
Arnal et al. (2019)
1 more