› 6mh8

X-ray diffraction
4.2Å resolution

High-viscosity injector-based Pink Beam Serial Crystallography of Micro-crystals at a Synchrotron Radiation Source

Released:
DOI: 10.2210/pdb6mh8/pdb
PDB entry 6mh8 coloured by chain, front view.
PDB entry 6mh8 coloured by chain, side view.
PDB entry 6mh8 coloured by chain, top view.
Source organisms:
Primary publication:
High-viscosity injector-based pink-beam serial crystallography of microcrystals at a synchrotron radiation source.
Martin-Garcia JM, Zhu L, Mendez D, Lee MY, Chun E, Li C, Hu H, Subramanian G, Kissick D, Ogata C, Henning R, Ishchenko A, Dobson Z, Zhang S, Weierstall U, Spence JCH, Fromme P, Zatsepin NA, Fischetti RF, Cherezov V, Liu W
IUCrJ 6 412-425 (2019)
PMID: 31098022

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142061 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosine receptor A2a; Soluble cytochrome b562 Chain: A
Molecule details ›
Chain: A
Length: 447 amino acids
Theoretical weight: 49.97 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P29274 (Residues: 2-208, 219-316; Coverage: 74%)
  • Canonical: P0ABE7 (Residues: 23-127; Coverage: 99%)
Gene names: ADORA2, ADORA2A, cybC
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ZMA 1 x ZMA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: C2221
Unit cell:
a: 40Å b: 179Å c: 142Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.254 0.25 0.288
Expression system: Spodoptera frugiperda

Quick links

Citations

2 review citations

Serial Crystallography for Structure-Based Drug Discovery.
Zhu et al. (2020)
1 more

6 mentions without citation

In Silico Drug Design for Purinergic GPCRs: Overview on Molecular Dynamics Applied to Adenosine and P2Y Receptors.
Salmaso et al. (2020)
5 more