› 6mly

X-ray diffraction
2.7Å resolution

Bifunctional GH43-CE Bacteroides eggerthii, BACEGG_01304

Released:
DOI: 10.2210/pdb6mly/pdb
PDB entry 6mly coloured by chain, front view.
PDB entry 6mly coloured by chain, side view.
PDB entry 6mly coloured by chain, top view.
Source organism: Bacteroides eggerthii
Primary publication:
Degradation of complex arabinoxylans by human colonic Bacteroidetes
Pereira GV, DAlessandro-Gabazza C, Farris J, Wefers D, Mackie R, Koropatkin NM, Gabazza EC, Cann I
Nat Commun (2021)

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123013 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycoside hydrolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 807 amino acids
Theoretical weight: 89.79 KDa
Source organism: Bacteroides eggerthii
Expression system: Escherichia coli
UniProt:
  • Canonical: E5WZQ7 (Residues: 1-757; Coverage: 98%)
Gene name: HMPREF1016_02163
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand ACT 4 x ACT
Ligand EDO 9 x EDO
Ligand NA 4 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: C2
Unit cell:
a: 254.734Å b: 93.31Å c: 214.039Å
α: 90° β: 123.38° γ: 90°
R-values:
R R work R free
0.222 0.22 0.269
Expression system: Escherichia coli

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