› 6mm6

X-ray diffraction
2.39Å resolution

Catalytic subunit of cAMP-dependent protein kinase A in complex with RyR2 phosphorylation domain (2699-2904)

Released:
DOI: 10.2210/pdb6mm6/pdb
PDB entry 6mm6 coloured by chain, front view.
PDB entry 6mm6 coloured by chain, side view.
PDB entry 6mm6 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
cAMP-dependent protein kinase A in complex with RyR2 peptide (2799-2810)
Haji-Ghassemi O, Yuchi Z, van Petegem F
Mol. Cell (2019)

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138536 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
cAMP-dependent protein kinase catalytic subunit alpha Chains: C, E
Molecule details ›
Chains: C, E
Length: 339 amino acids
Theoretical weight: 39.6 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P05132 (Residues: 16-351; Coverage: 96%)
Gene names: Pkaca, Prkaca
Sequence domains: Protein kinase domain
Structure domains:
Ryanodine receptor 2 Chains: D, F
Molecule details ›
Chains: D, F
Length: 209 amino acids
Theoretical weight: 24.26 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: E9Q401 (Residues: 2699-2904; Coverage: 4%)
Gene name: Ryr2
Sequence domains: RyR domain
Structure domains: Globin-like

Ligands and Environments

5 bound ligands:
Ligand ACT 1 x ACT
Ligand ANP 2 x ANP
Ligand PGE 1 x PGE
Ligand CL 3 x CL
Ligand EDO 1 x EDO
2 modified residues:
Ligand TPO 2 x TPO
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08B1-1
Spacegroup: P1
Unit cell:
a: 58.451Å b: 74.944Å c: 88.69Å
α: 105.51° β: 90.14° γ: 94.39°
R-values:
R R work R free
0.225 0.224 0.261
Expression system: Escherichia coli

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