› 6mpx

X-ray diffraction
1.9Å resolution

Twelve chloride ions induce formation and stabilize the NC1 hexamer of collagen IV assembled from transition state trimers

Released:
DOI: 10.2210/pdb6mpx/pdb
PDB entry 6mpx coloured by chain, front view.
PDB entry 6mpx coloured by chain, side view.
PDB entry 6mpx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A chloride ring is an ancient evolutionary innovation mediating the assembly of the collagen IV scaffold of basement membranes.
Pedchenko V, Bauer R, Pokidysheva EN, Al-Shaer A, Forde NR, Fidler AL, Hudson BG, Boudko SP
J Biol Chem 294 7968-7981 (2019)
PMID: 30923125

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135862 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arresten; Canstatin Chain: A
Molecule details ›
Chain: A
Length: 693 amino acids
Theoretical weight: 76.31 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P02462 (Residues: 1438-1666; Coverage: 14%)
  • Canonical: P08572 (Residues: 1489-1709; Coverage: 13%)
Gene names: COL4A1, COL4A2
Sequence domains: C-terminal tandem repeated domain in type 4 procollagen

Ligands and Environments

6 bound ligands:
Ligand CL 6 x CL
Ligand SO4 8 x SO4
Ligand PEG 1 x PEG
Ligand PGE 1 x PGE
Ligand P6G 1 x P6G
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P41212
Unit cell:
a: 121.16Å b: 121.16Å c: 106.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.149 0.148 0.177
Expression system: Cricetulus griseus

Quick links

Citations

3 review citations

Cl- as a bona fide signaling ion.
Lüscher et al. (2020)
2 more

3 mentions without citation

A chloride ring is an ancient evolutionary innovation mediating the assembly of the collagen IV scaffold of basement membranes.
Pedchenko et al. (2019)
2 more