› 6muv

Electron Microscopy
3.8Å resolution

The structure of the Plasmodium falciparum 20S proteasome in complex with two PA28 activators

Released:
DOI: 10.2210/pdb6muv/pdb
PDB entry 6muv coloured by chain, front view.
PDB entry 6muv coloured by chain, side view.
PDB entry 6muv coloured by chain, top view.
Source organism: Plasmodium falciparum 3D7
Primary publication:
The structure of the PA28-20S proteasome complex from Plasmodium falciparum and implications for proteostasis.
Xie SC, Metcalfe RD, Hanssen E, Yang T, Gillett DL, Leis AP, Morton CJ, Kuiper MJ, Parker MW, Spillman NJ, Wong W, Tsu C, Dick LR, Griffin MDW, Tilley L
Nat Microbiol 4 1990-2000 (2019)
PMID: 31384003
Related structures: EMD-9257

Function and Biology Details

Reaction catalysed: 
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 42-mer (preferred)
PDBe Complex ID:
PDB-CPX-111605 (preferred)
Entry contents:
15 distinct polypeptide molecules
Macromolecules (15 distinct):
Proteasome alpha-type subunits domain-containing protein Chains: A, O
Molecule details ›
Chains: A, O
Length: 260 amino acids
Theoretical weight: 29.53 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IAR3 (Residues: 1-260; Coverage: 100%)
Gene name: PF3D7_0807500
Sequence domains:
Proteasome alpha-type subunits domain-containing protein Chains: B, P
Molecule details ›
Chains: B, P
Length: 235 amino acids
Theoretical weight: 26.56 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: C6KST3 (Residues: 1-235; Coverage: 100%)
Gene name: PF3D7_0608500
Sequence domains:
Proteasome subunit alpha type Chains: C, Q
Molecule details ›
Chains: C, Q
Length: 246 amino acids
Theoretical weight: 27.98 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IDG3 (Residues: 1-246; Coverage: 100%)
Gene name: PF3D7_1353800
Sequence domains:
Proteasome subunit alpha type Chains: D, R
Molecule details ›
Chains: D, R
Length: 241 amino acids
Theoretical weight: 27.26 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IDG2 (Residues: 1-241; Coverage: 100%)
Gene name: PF3D7_1353900
Sequence domains:
Proteasome subunit alpha type Chains: E, S
Molecule details ›
Chains: E, S
Length: 256 amino acids
Theoretical weight: 28.42 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IBI3 (Residues: 1-256; Coverage: 100%)
Gene name: PF3D7_0727400
Sequence domains:
Proteasome alpha-type subunits domain-containing protein Chains: F, T
Molecule details ›
Chains: F, T
Length: 254 amino acids
Theoretical weight: 28.87 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IK90 (Residues: 1-254; Coverage: 100%)
Gene name: PF3D7_1474800
Sequence domains:
Proteasome alpha-type subunits domain-containing protein Chains: G, U
Molecule details ›
Chains: G, U
Length: 252 amino acids
Theoretical weight: 29.32 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: O77396 (Residues: 1-252; Coverage: 100%)
Gene name: PF3D7_0317000
Sequence domains:
Proteasome subunit beta type-6, putative Chains: H, V
Molecule details ›
Chains: H, V
Length: 252 amino acids
Theoretical weight: 29.14 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8I0U7 (Residues: 31-282; Coverage: 89%)
Gene name: PF3D7_0931800
Sequence domains: Proteasome subunit
Proteasome subunit beta Chains: I, W
Molecule details ›
Chains: I, W
Length: 229 amino acids
Theoretical weight: 25.1 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8I6T3 (Residues: 42-270; Coverage: 85%)
Gene name: PF3D7_1328100
Sequence domains: Proteasome subunit
Proteasome subunit beta Chains: J, X
Molecule details ›
Chains: J, X
Length: 218 amino acids
Theoretical weight: 24.53 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8I261 (Residues: 1-218; Coverage: 100%)
Gene name: PF3D7_0108000
Sequence domains: Proteasome subunit
Proteasome subunit beta Chains: K, Y
Molecule details ›
Chains: K, Y
Length: 195 amino acids
Theoretical weight: 22.89 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IKC9 (Residues: 1-195; Coverage: 100%)
Gene name: PF3D7_1470900
Sequence domains: Proteasome subunit
Proteasome subunit beta Chains: L, Z
Molecule details ›
Chains: L, Z
Length: 211 amino acids
Theoretical weight: 23.62 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IJT1 (Residues: 61-271; Coverage: 78%)
Gene name: PF3D7_1011400
Sequence domains: Proteasome subunit
20S proteasome beta-6 subunit Chains: M, a
Molecule details ›
Chains: M, a
Length: 240 amino acids
Theoretical weight: 27.3 KDa
Source organism: Plasmodium falciparum 3D7
Proteasome subunit beta Chains: N, b
Molecule details ›
Chains: N, b
Length: 265 amino acids
Theoretical weight: 30.91 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q7K6A9 (Residues: 1-265; Coverage: 100%)
Gene name: PF3D7_0803800
Sequence domains: Proteasome subunit
Proteasome activator PA28 C-terminal domain-containing protein Chains: c, d, e, f, g, h, i, j, k, l, m, n, o, p
Molecule details ›
Chains: c, d, e, f, g, h, i, j, k, l, m, n, o, p
Length: 279 amino acids
Theoretical weight: 33.18 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8I374 (Residues: 1-279; Coverage: 100%)
Gene name: PF3D7_0907700
Sequence domains: Proteasome activator pa28 beta subunit

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-9257
Expression system: Escherichia coli

Quick links

Citations

4 review citations

MalDA, Accelerating Malaria Drug Discovery.
Yang et al. (2021)
3 more

2 mentions without citation

Complementarity of Hydrogen/Deuterium Exchange Mass Spectrometry and Cryo-Electron Microscopy.
Engen et al. (2020)
1 more