› 6mv5

X-ray diffraction
2.1Å resolution

Anti-PCSK9 fab 6E2 bound to the N-terminal peptide from PCSK9 (E32K)

Released:
DOI: 10.2210/pdb6mv5/pdb
PDB entry 6mv5 coloured by chain, front view.
PDB entry 6mv5 coloured by chain, side view.
PDB entry 6mv5 coloured by chain, top view.
Source organisms:
Primary publication:
Identification of a Helical Segment within the Intrinsically Disordered Region of the PCSK9 Prodomain.
Ultsch M, Li W, Eigenbrot C, Di Lello P, Lipari MT, Gerhardy S, AhYoung AP, Quinn J, Franke Y, Chen Y, Kong Beltran M, Peterson A, Kirchhofer D
J Mol Biol 431 885-903 (2019)
PMID: 30653992

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-231517 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Anti-PCSK9 fab 6E2 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 224 amino acids
Theoretical weight: 24.26 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Ig-like domain-containing protein Chain: L
Molecule details ›
Chain: L
Length: 219 amino acids
Theoretical weight: 24.04 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A097PUG4 (Residues: 21-51, 52-238; Coverage: 100%)
Sequence domains:
Structure domains: Immunoglobulins
Proprotein convertase subtilisin/kexin type 9 Chain: P
Molecule details ›
Chain: P
Length: 23 amino acids
Theoretical weight: 2.49 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q8NBP7 (Residues: 32-53; Coverage: 3%)
Gene names: NARC1, PCSK9, PSEC0052

Ligands and Environments

3 bound ligands:
Ligand ZN 4 x ZN
Ligand CL 1 x CL
Ligand SO4 2 x SO4
1 modified residue:
Ligand NEP 1 x NEP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21212
Unit cell:
a: 70.449Å b: 100.42Å c: 86.59Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.21 0.238
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 citation in other articles

Ser-Phosphorylation of PCSK9 (Proprotein Convertase Subtilisin-Kexin 9) by Fam20C (Family With Sequence Similarity 20, Member C) Kinase Enhances Its Ability to Degrade the LDLR (Low-Density Lipoprotein Receptor).
Ben Djoudi Ouadda et al. (2019)
3 more