› 6mz3

X-ray diffraction
1.09Å resolution

mCherry pH sensitive mutant - M66T (mCherryTYG)

Released:
DOI: 10.2210/pdb6mz3/pdb
Model geometry
Fit model/data
PDB entry 6mz3 coloured by chain, front view.
PDB entry 6mz3 coloured by chain, side view.
PDB entry 6mz3 coloured by chain, top view.
Source organism: Discosoma sp.
Primary publication:
Quantifying Acute Fuel and Respiration Dependent pH Homeostasis in Live Cells Using the mCherryTYG Mutant as a Fluorescence Lifetime Sensor.
Haynes EP, Rajendran M, Henning CK, Mishra A, Lyon AM, Tantama M
Anal Chem 91 8466-8475 (2019)
PMID: 31247720

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112322 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 268 amino acids
Theoretical weight: 30.56 KDa
Source organism: Discosoma sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: D1MPT3 (Residues: 1-236; Coverage: 100%)
Gene names: PAmCherry, PAmCherry1
Sequence domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRO 1 x CRO

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21
Unit cell:
a: 48.806Å b: 42.782Å c: 61.067Å
α: 90° β: 112.2° γ: 90°
R-values:
R R work R free
0.167 0.167 0.178
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Probing organoid metabolism using fluorescence lifetime imaging microscopy (FLIM): The next frontier of drug discovery and disease understanding.
Barroso et al. (2023)
1 more

1 mention without citation

mCherry contains a fluorescent protein isoform that interferes with its reporter function.
Fages-Lartaud et al. (2022)