› 6n1h

Electron Microscopy
3.17Å resolution

Cryo-EM structure of ASC-CARD filament

Released:
DOI: 10.2210/pdb6n1h/pdb
PDB entry 6n1h coloured by chain, front view.
PDB entry 6n1h coloured by chain, side view.
PDB entry 6n1h coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM structures of ASC and NLRC4 CARD filaments reveal a unified mechanism of nucleation and activation of caspase-1.
Li Y, Fu TM, Lu A, Witt K, Ruan J, Shen C, Wu H
Proc Natl Acad Sci U S A 115 10845-10852 (2018)
PMID: 30279182
Related structures: EMD-8902

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193951 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Apoptosis-associated speck-like protein containing a CARD Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Length: 83 amino acids
Theoretical weight: 9.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ULZ3 (Residues: 112-194; Coverage: 43%)
Gene names: ASC, CARD5, PYCARD, TMS1
Sequence domains: Caspase recruitment domain
Structure domains: Death Domain, Fas

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.17Å
Relevant EMDB volumes: EMD-8902
Expression system: Escherichia coli

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Citations

31 review citations

Inflammasome activation and regulation: toward a better understanding of complex mechanisms.
Zheng et al. (2020)
30 more

8 mentions without citation

Emerging chemistry and biology in protein glutathionylation.
Kukulage et al. (2022)
7 more