6n1p

Electron Microscopy
6.35Å resolution

Dihedral oligomeric complex of GyrA N-terminal fragment with DNA, solved by cryoEM in C2 symmetry

Released:

Function and Biology Details

Reaction catalysed:
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-120310 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA gyrase subunit A Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 511 amino acids
Theoretical weight: 57.98 KDa
Source organism: Streptococcus pneumoniae G54
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8DPM2 (Residues: 1-487; Coverage: 59%)
Gene names: gyrA, spr1099
Sequence domains: DNA gyrase/topoisomerase IV, subunit A
DNA (44-MER) Chain: I
Molecule details ›
Chain: I
Length: 44 nucleotides
Theoretical weight: 13.64 KDa
Source organism: Cloning vector pBR322
Expression system: Not provided
DNA (44-MER) Chain: J
Molecule details ›
Chain: J
Length: 44 nucleotides
Theoretical weight: 13.46 KDa
Source organism: Cloning vector pBR322
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.35Å
Relevant EMDB volumes: EMD-9316
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided