› 6n1z

X-ray diffraction
2.7Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-135439 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Importin-9 Chains: A, D
Molecule details ›
Chains: A, D
Length: 1041 amino acids
Theoretical weight: 116.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96P70 (Residues: 1-1041; Coverage: 100%)
Gene names: HSPC273, IMP9, IPO9, KIAA1192, RANBP9
Sequence domains: Importin-beta N-terminal domain
Histone H2A type 1 Chains: B, E
Molecule details ›
Chains: B, E
Length: 130 amino acids
Theoretical weight: 14.11 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P06897 (Residues: 1-130; Coverage: 100%)
Sequence domains:
Structure domains: Histone, subunit A
Histone H2B 1.1 Chains: C, F
Molecule details ›
Chains: C, F
Length: 123 amino acids
Theoretical weight: 13.66 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P02281 (Residues: 5-126; Coverage: 97%)
Sequence domains: Core histone H2A/H2B/H3/H4
Structure domains: Histone, subunit A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 127.424Å b: 223.289Å c: 131.833Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.209 0.238
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

6 review citations

Karyopherin-mediated nucleocytoplasmic transport.
Wing et al. (2022)
5 more

4 mentions without citation

Nuclear import of histones.
Bernardes et al. (2020)
3 more