› 6n2m

Solution NMR

NMR solution structure of the homodimeric, autoinhibited state of the CARD9 CARD and first coiled-coil

Released:
DOI: 10.2210/pdb6n2m/pdb
PDB entry 6n2m coloured by chain, ensemble of 20 models, front view.
PDB entry 6n2m coloured by chain, ensemble of 20 models, side view.
PDB entry 6n2m coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Structures of autoinhibited and polymerized forms of CARD9 reveal mechanisms of CARD9 and CARD11 activation.
Holliday MJ, Witt A, Rodríguez Gama A, Walters BT, Arthur CP, Halfmann R, Rohou A, Dueber EC, Fairbrother WJ
Nat Commun 10 3070 (2019)
PMID: 31296852

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190538 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Caspase recruitment domain-containing protein 9 Chains: A, B
Molecule details ›
Chains: A, B
Length: 142 amino acids
Theoretical weight: 16.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H257 (Residues: 2-142; Coverage: 26%)
Gene name: CARD9
Sequence domains: Caspase recruitment domain

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 45%
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

CARD9 Signaling, Inflammation, and Diseases.
Liu et al. (2022)
5 more

3 mentions without citation

Assembly of platforms for signal transduction in the new era: dimerization, helical filament assembly, and beyond.
Ha et al. (2020)
2 more