› 6n2w

X-ray diffraction
2.71Å resolution

The structure of Stable-5-Lipoxygenase bound to NDGA

Released:
DOI: 10.2210/pdb6n2w/pdb
PDB entry 6n2w coloured by chain, front view.
PDB entry 6n2w coloured by chain, side view.
PDB entry 6n2w coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and mechanistic insights into 5-lipoxygenase inhibition by natural products.
Gilbert NC, Gerstmeier J, Schexnaydre EE, Börner F, Garscha U, Neau DB, Werz O, Newcomer ME
Nat Chem Biol 16 783-790 (2020)
PMID: 32393899

Function and Biology Details

Reaction catalysed: 
(1a) arachidonate + O(2) = (5S,6E,8Z,11Z,14Z)-5-hydroperoxyicosa-6,8,11,14-tetraenoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140817 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyunsaturated fatty acid 5-lipoxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 691 amino acids
Theoretical weight: 79.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09917 (Residues: 1-674; Coverage: 100%)
Gene names: ALOX5, LOG5
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand FE2 2 x FE2
Ligand 30Z 1 x 30Z
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 48.152Å b: 204.358Å c: 76.712Å
α: 90° β: 99.906° γ: 90°
R-values:
R R work R free
0.216 0.213 0.263
Expression system: Escherichia coli

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Citations

12 review citations

Boswellic acids/Boswellia serrata extract as a potential COVID-19 therapeutic agent in the elderly.
Gomaa et al. (2021)
11 more

35 mentions without citation

Structural and mechanistic insights into 5-lipoxygenase inhibition by natural products.
Gilbert et al. (2020)
34 more