› 6n3h

X-ray diffraction
2.6Å resolution

Crystal structure of Kelch domain of the human NS1 binding protein

Released:
DOI: 10.2210/pdb6n3h/pdb
PDB entry 6n3h coloured by chain, front view.
PDB entry 6n3h coloured by chain, side view.
PDB entry 6n3h coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural-functional interactions of NS1-BP protein with the splicing and mRNA export machineries for viral and host gene expression.
Zhang K, Shang G, Padavannil A, Wang J, Sakthivel R, Chen X, Kim M, Thompson MG, García-Sastre A, Lynch KW, Chen ZJ, Chook YM, Fontoura BMA
Proc Natl Acad Sci U S A 115 E12218-E12227 (2018)
PMID: 30538201

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195397 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Influenza virus NS1A-binding protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 331 amino acids
Theoretical weight: 36.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q9Y6Y0 (Residues: 321-642; Coverage: 50%)
Gene names: ARA3, FLARA3, HSPC068, IVNS1ABP, KIAA0850, KLHL39, NS1, NS1BP
Sequence domains: Kelch motif

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 77.196Å b: 61.885Å c: 87.339Å
α: 90° β: 95.18° γ: 90°
R-values:
R R work R free
0.212 0.211 0.234
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

5 review citations

Environmental Risk Assessment of Recombinant Viral Vector Vaccines against SARS-Cov-2.
Baldo et al. (2021)
4 more

3 mentions without citation

Challenges and Limitations of Targeting the Keap1-Nrf2 Pathway for Neurotherapeutics: Bach1 De-Repression to the Rescue.
Hushpulian et al. (2021)
2 more