› 6n3i

X-ray diffraction
3.69Å resolution

Crystal structure of a double Trp XylE mutants (G58W/L315W)

Released:
DOI: 10.2210/pdb6n3i/pdb
PDB entry 6n3i coloured by chain, front view.
PDB entry 6n3i coloured by chain, side view.
PDB entry 6n3i coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Engineered XylE as a tool for mechanistic investigation and ligand discovery of the glucose transporters GLUTs.
Jiang X, Wu J, Ke M, Zhang S, Yuan Y, Lin JY, Yan N
Cell Discov 5 14 (2019)
PMID: 30854221

Function and Biology Details

Reaction catalysed: 
A n-alkanal + NAD(P)(+) = an alk-2-enal + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142832 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-xylose-proton symporter Chain: A
Molecule details ›
Chain: A
Length: 518 amino acids
Theoretical weight: 56.77 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AGF4 (Residues: 1-491; Coverage: 100%)
Gene names: JW3991, b4031, xylE
Sequence domains: Sugar (and other) transporter
Structure domains: MFS general substrate transporter like domains

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 89.076Å b: 95.482Å c: 106.837Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.293 0.291 0.33
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Highlighting membrane protein structure and function: A celebration of the Protein Data Bank.
Li et al. (2021)
8 other articles

4 mentions without citation

Engineered XylE as a tool for mechanistic investigation and ligand discovery of the glucose transporters GLUTs.
Jiang et al. (2019)
3 more