› 6n4u

Electron crystallography
2.75Å resolution

MicroED structure of Proteinase K at 2.75A resolution from a single milled crystal.

Released:
DOI: 10.2210/pdb6n4u/pdb
PDB entry 6n4u coloured by chain, front view.
PDB entry 6n4u coloured by chain, side view.
PDB entry 6n4u coloured by chain, top view.
Source organism: Parengyodontium album
Primary publication:
Collection of Continuous Rotation MicroED Data from Ion Beam-Milled Crystals of Any Size.
Martynowycz MW, Zhao W, Hattne J, Jensen GJ, Gonen T
Structure 27 545-548.e2 (2019)
PMID: 30661853
Related structures: EMD-0343

Function and Biology Details

Reaction catalysed: 
Hydrolysis of keratin, and of other proteins with subtilisin-like specificity. Hydrolyzes peptide amides.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139275 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proteinase K Chain: A
Molecule details ›
Chain: A
Length: 279 amino acids
Theoretical weight: 28.96 KDa
Source organism: Parengyodontium album
Expression system: Parengyodontium album
UniProt:
  • Canonical: P06873 (Residues: 106-384; Coverage: 76%)
Gene name: PROK
Sequence domains: Subtilase family
Structure domains: Peptidase S8/S53 domain

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.75Å
Relevant EMDB volumes: EMD-0343
Expression system: Parengyodontium album

Quick links

Citations

7 review citations

The cryo-EM method microcrystal electron diffraction (MicroED).
Nannenga et al. (2019)
6 more

2 mentions without citation

Electron Diffraction of 3D Molecular Crystals.
Saha et al. (2022)
1 more