› 6n4y

X-ray diffraction
3.26Å resolution

Metabotropic Glutamate Receptor 5 Extracellular Domain with Nb43

Released:
DOI: 10.2210/pdb6n4y/pdb
PDB entry 6n4y coloured by chain, front view.
PDB entry 6n4y coloured by chain, side view.
PDB entry 6n4y coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into the activation of metabotropic glutamate receptors.
Koehl A, Hu H, Feng D, Sun B, Zhang Y, Robertson MJ, Chu M, Kobilka TS, Laeremans T, Steyaert J, Tarrasch J, Dutta S, Fonseca R, Weis WI, Mathiesen JM, Skiniotis G, Kobilka BK
Nature 566 79-84 (2019)
PMID: 30675062

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-231806 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (6 distinct):
Metabotropic glutamate receptor 5 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 596 amino acids
Theoretical weight: 67.4 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P41594 (Residues: 21-571; Coverage: 46%)
Gene names: GPRC1E, GRM5, MGLUR5
Sequence domains:
Nanobody 43 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 123 amino acids
Theoretical weight: 13.35 KDa
Source organism: Lama glama
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC, BMA, MAN
Carbohydrate polymer
1 bound ligand:
Ligand NAG 4 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21
Unit cell:
a: 95.528Å b: 158.389Å c: 111.963Å
α: 90° β: 101.93° γ: 90°
R-values:
R R work R free
0.201 0.199 0.251
Expression systems:
  • Trichoplusia ni
  • Escherichia coli BL21(DE3)

Quick links

Citations

35 review citations

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
34 more

3 mentions without citation

G protein-coupled receptors in neurodegenerative diseases and psychiatric disorders.
Wong et al. (2023)
2 more