› 6n5w

X-ray diffraction
2.15Å resolution

Crystal structure of the Ca2+/CaM complex with independent peptides of Kv7.4 (KCNQ4) A & B domains

Released:
DOI: 10.2210/pdb6n5w/pdb
PDB entry 6n5w coloured by chain, front view.
PDB entry 6n5w coloured by chain, side view.
PDB entry 6n5w coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A mutually induced conformational fit underlies Ca2+-directed interactions between calmodulin and the proximal C terminus of KCNQ4 K+ channels.
Archer CR, Enslow BT, Taylor AB, De la Rosa V, Bhattacharya A, Shapiro MS
J Biol Chem 294 6094-6112 (2019)
PMID: 30808708

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143930 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Potassium voltage-gated channel subfamily KQT member 4 Chain: A
Molecule details ›
Chain: A
Length: 27 amino acids
Theoretical weight: 3.22 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P56696 (Residues: 336-362; Coverage: 4%)
Gene name: KCNQ4
Potassium voltage-gated channel subfamily KQT member 4 Chain: B
Molecule details ›
Chain: B
Length: 26 amino acids
Theoretical weight: 3.07 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P56696 (Residues: 524-549; Coverage: 4%)
Gene name: KCNQ4
Calmodulin-1 Chain: C
Molecule details ›
Chain: C
Length: 149 amino acids
Theoretical weight: 16.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0DP23 (Residues: 1-149; Coverage: 100%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21212
Unit cell:
a: 43.51Å b: 130.57Å c: 36.16Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.232 0.274
Expression systems:
  • Not provided
  • Escherichia coli BL21

Quick links

Citations

1 review citation

Pharmacological Manipulation of K v 7 Channels as a New Therapeutic Tool for Multiple Brain Disorders.
Vigil et al. (2020)
5 other articles

2 mentions without citation

Atomistic Insights of Calmodulin Gating of Complete Ion Channels.
Núñez et al. (2020)
1 more