› 6n7f

X-ray diffraction
1.9Å resolution

1.90 Angstrom Resolution Crystal Structure of Glutathione Reductase from Streptococcus pyogenes in Complex with FAD.

Released:
DOI: 10.2210/pdb6n7f/pdb
PDB entry 6n7f coloured by chain, front view.
PDB entry 6n7f coloured by chain, side view.
PDB entry 6n7f coloured by chain, top view.
Source organism: Streptococcus pyogenes serotype M1
Entry authors: Minasov G, Shuvalova L, Shabalin IG, Grabowski M, Olphie A, Cardona-Correa A, Anderson WF, Satchell KJF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
2 glutathione + NADP(+) = glutathione disulfide + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189441 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione reductase (GR) Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 453 amino acids
Theoretical weight: 49.25 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9A0E2 (Residues: 1-450; Coverage: 100%)
Gene names: SPy_0813, gor
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 4 x FAD
5 bound ligands:
Ligand RBF 1 x RBF
Ligand BTB 4 x BTB
Ligand BCT 1 x BCT
Ligand CL 17 x CL
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P1
Unit cell:
a: 76.4Å b: 86.65Å c: 86.67Å
α: 67.13° β: 74.15° γ: 74.11°
R-values:
R R work R free
0.171 0.169 0.21
Expression system: Escherichia coli BL21(DE3)

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