› 6n84

X-ray diffraction
1.75Å resolution

MBP-fusion protein of transducin-alpha residues 327-350

Released:
DOI: 10.2210/pdb6n84/pdb
PDB entry 6n84 coloured by chain, front view.
PDB entry 6n84 coloured by chain, side view.
PDB entry 6n84 coloured by chain, top view.
Source organisms:
Primary publication:
Structural underpinnings of Ric8A function as a G-protein α-subunit chaperone and guanine-nucleotide exchange factor.
Srivastava D, Gakhar L, Artemyev NO
Nat Commun 10 3084 (2019)
PMID: 31300652

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142521 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Guanine nucleotide-binding protein G(t) subunit alpha-2; Maltose/maltodextrin-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 415 amino acids
Theoretical weight: 45.45 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
  • Canonical: P19087 (Residues: 331-354; Coverage: 7%)
Gene names: GNAT2, GNATC, JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand SO4 8 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P63
Unit cell:
a: 83.07Å b: 83.07Å c: 109.229Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.158 0.156 0.185
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Strategies towards Targeting Gαi/s Proteins: Scanning of Protein-Protein Interaction Sites To Overcome Inaccessibility.
Nubbemeyer et al. (2021)
10 other articles