› 6nbc

Electron Microscopy
2.8Å resolution

human methemoglobin state 1 determined using single-particle cryo-EM at 200 keV

Released:
DOI: 10.2210/pdb6nbc/pdb
PDB entry 6nbc coloured by chain, ensemble of 10 models, front view.
PDB entry 6nbc coloured by chain, ensemble of 10 models, side view.
PDB entry 6nbc coloured by chain, ensemble of 10 models, top view.
Source organism: Homo sapiens
Primary publication:
High-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM.
Herzik MA, Wu M, Lander GC
Nat Commun 10 1032 (2019)
PMID: 30833564
Related structures: EMD-0407

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159519 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hemoglobin subunit alpha Chains: A, C
Molecule details ›
Chains: A, C
Length: 140 amino acids
Theoretical weight: 14.99 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P69905 (Residues: 2-141; Coverage: 99%)
Gene names: HBA1, HBA2
Sequence domains: Globin
Structure domains: Globins
Hemoglobin subunit beta Chains: B, D
Molecule details ›
Chains: B, D
Length: 143 amino acids
Theoretical weight: 15.46 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P68871 (Residues: 2-144; Coverage: 97%)
Gene name: HBB
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

1 bound ligand:
Ligand HEM 4 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-0407

Quick links

Citations

22 review citations

Understanding the invisible hands of sample preparation for cryo-EM.
Weissenberger et al. (2021)
21 more

2 mentions without citation

Statins affect cancer cell plasticity with distinct consequences for tumor progression and metastasis.
Dorsch et al. (2021)
1 more