› 6ndj

X-ray diffraction
2.27Å resolution

Crystal structure of human NLRP6 PYD domain with MBP fusion

Released:
DOI: 10.2210/pdb6ndj/pdb
PDB entry 6ndj coloured by chain, front view.
PDB entry 6ndj coloured by chain, side view.
PDB entry 6ndj coloured by chain, top view.
Source organisms:
Primary publication:
Molecular mechanism for NLRP6 inflammasome assembly and activation.
Shen C, Lu A, Xie WJ, Ruan J, Negro R, Egelman EH, Fu TM, Wu H
Proc Natl Acad Sci U S A 116 2052-2057 (2019)
PMID: 30674671

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142544 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Maltose/maltodextrin-binding periplasmic protein; NACHT, LRR and PYD domains-containing protein 6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 473 amino acids
Theoretical weight: 52.37 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 29-387; Coverage: 97%)
  • Canonical: P59044 (Residues: 14-106; Coverage: 10%)
Gene names: JW3994, NALP6, NLRP6, PYPAF5, b4034, malE
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P1
Unit cell:
a: 38.283Å b: 65.364Å c: 102.362Å
α: 95.63° β: 92.13° γ: 106.59°
R-values:
R R work R free
0.19 0.187 0.229
Expression system: Escherichia coli

Quick links

Citations

27 review citations

Inflammasome activation and regulation: toward a better understanding of complex mechanisms.
Zheng et al. (2020)
26 more

2 mentions without citation

Assembly of platforms for signal transduction in the new era: dimerization, helical filament assembly, and beyond.
Ha et al. (2020)
1 more