› 6ne1

X-ray diffraction
3.01Å resolution

Designed repeat protein in complex with Fz4

Released:
DOI: 10.2210/pdb6ne1/pdb
PDB entry 6ne1 coloured by chain, front view.
PDB entry 6ne1 coloured by chain, side view.
PDB entry 6ne1 coloured by chain, top view.
Source organisms:
Primary publication:
Receptor subtype discrimination using extensive shape complementary designed interfaces.
Dang LT, Miao Y, Ha A, Yuki K, Park K, Janda CY, Jude KM, Mohan K, Ha N, Vallon M, Yuan J, Vilches-Moure JG, Kuo CJ, Garcia KC, Baker D
Nat Struct Mol Biol 26 407-414 (2019)
PMID: 31086346

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167120 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Frizzled-4 Chain: A
Molecule details ›
Chain: A
Length: 120 amino acids
Theoretical weight: 13.62 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9ULV1 (Residues: 42-161; Coverage: 24%)
Gene name: FZD4
Sequence domains: Fz domain
Structure domains: Frizzled cysteine-rich domain
Pfs, NACHT and Ankyrin domain protein Chain: B
Molecule details ›
Chain: B
Length: 192 amino acids
Theoretical weight: 19.95 KDa
Source organism: Aspergillus fumigatus Af293
Expression system: Escherichia coli
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Unit cell:
a: 51.846Å b: 62.611Å c: 86.217Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.234 0.293
Expression systems:
  • Trichoplusia ni
  • Escherichia coli

Quick links

Citations

3 review citations

Protein Assembly by Design.
Zhu et al. (2021)
2 more

1 mention without citation

Receptor subtype discrimination using extensive shape complementary designed interfaces.
Dang et al. (2019)