› 6nie

X-ray diffraction
1.95Å resolution

BesD with Fe(II), chloride, and alpha-ketoglutarate

Released:
DOI: 10.2210/pdb6nie/pdb
PDB entry 6nie coloured by chain, front view.
PDB entry 6nie coloured by chain, side view.
PDB entry 6nie coloured by chain, top view.
Source organism: Streptomyces cattleya NRRL 8057 = DSM 46488
Primary publication:
A family of radical halogenases for the engineering of amino-acid-based products.
Neugebauer ME, Sumida KH, Pelton JG, McMurry JL, Marchand JA, Chang MCY
Nat Chem Biol 15 1009-1016 (2019)
PMID: 31548692

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124646 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lysine 4-chlorinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 255 amino acids
Theoretical weight: 29.32 KDa
Source organism: Streptomyces cattleya NRRL 8057 = DSM 46488
Expression system: Escherichia coli
UniProt:
  • Canonical: G8XHD5 (Residues: 2-242; Coverage: 100%)
Gene names: SCATT_p06880, besD

Ligands and Environments

5 bound ligands:
Ligand LYS 4 x LYS
Ligand FE2 4 x FE2
Ligand CL 4 x CL
Ligand AKG 4 x AKG
Ligand FE 1 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P1
Unit cell:
a: 61.358Å b: 68.916Å c: 79.702Å
α: 115.36° β: 91.57° γ: 112.95°
R-values:
R R work R free
0.179 0.177 0.216
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Biocatalysis: Enzymatic Synthesis for Industrial Applications.
Wu et al. (2021)
4 more