› 6nin

X-ray diffraction
3.6Å resolution

Rhodobacter sphaeroides bc1 with STIGMATELLIN A

Released:
DOI: 10.2210/pdb6nin/pdb
PDB entry 6nin coloured by chain, front view.
PDB entry 6nin coloured by chain, side view.
PDB entry 6nin coloured by chain, top view.
Source organism: Cereibacter sphaeroides 2.4.1
Primary publication:
Crystal structure of bacterial cytochrome bc 1 in complex with azoxystrobin reveals a conformational switch of the Rieske iron-sulfur protein subunit.
Esser L, Zhou F, Yu CA, Xia D
J Biol Chem 294 12007-12019 (2019)
PMID: 31182483

Function and Biology Details

Reaction catalysed: 
Quinol + 2 ferricytochrome c(Side 2) = quinone + 2 ferrocytochrome c(Side 2) + 2 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-174589 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cytochrome b Chains: A, E, K, O, S, W
Molecule details ›
Chains: A, E, K, O, S, W
Length: 445 amino acids
Theoretical weight: 50.12 KDa
Source organism: Cereibacter sphaeroides 2.4.1
UniProt:
  • Canonical: Q3IY10 (Residues: 1-445; Coverage: 100%)
Gene names: RSP_1395, fbcB
Sequence domains:
Cytochrome c1 Chains: B, F, L, P, T, X
Molecule details ›
Chains: B, F, L, P, T, X
Length: 272 amino acids
Theoretical weight: 29.59 KDa
Source organism: Cereibacter sphaeroides 2.4.1
UniProt:
  • Canonical: Q3IY11 (Residues: 23-285; Coverage: 100%)
Gene names: RSP_1394, fbcC
Sequence domains: Cytochrome C1 family
Ubiquinol-cytochrome c reductase iron-sulfur subunit Chains: C, G, M, Q, U, Y
Molecule details ›
Chains: C, G, M, Q, U, Y
Length: 187 amino acids
Theoretical weight: 19.9 KDa
Source organism: Cereibacter sphaeroides 2.4.1
UniProt:
  • Canonical: Q3IY09 (Residues: 1-187; Coverage: 100%)
Gene names: RSP_1396, fbcF
Sequence domains:

Ligands and Environments

7 bound ligands:
Ligand HEM 12 x HEM
Ligand SMA 6 x SMA
Ligand 6PE 3 x 6PE
Ligand HEC 6 x HEC
Ligand SR 6 x SR
Ligand BOG 6 x BOG
Ligand FES 6 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 356.661Å b: 145.752Å c: 162.223Å
α: 90° β: 104.97° γ: 90°
R-values:
R R work R free
0.25 0.249 0.28

Quick links

Citations

5 review citations

Catalytic Reactions and Energy Conservation in the Cytochrome bc1 and b6f Complexes of Energy-Transducing Membranes.
Sarewicz et al. (2021)
4 more

1 mention without citation

Crystal structure of bacterial cytochrome bc 1 in complex with azoxystrobin reveals a conformational switch of the Rieske iron-sulfur protein subunit.
Esser et al. (2019)