› 6nlq

X-ray diffraction
1.15Å resolution

Human Mitochondrial Alanyl-tRNA Synthetase C-terminal domain

Released:
DOI: 10.2210/pdb6nlq/pdb
PDB entry 6nlq coloured by chain, front view.
PDB entry 6nlq coloured by chain, side view.
PDB entry 6nlq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Relaxed sequence constraints favor mutational freedom in idiosyncratic metazoan mitochondrial tRNAs.
Kuhle B, Chihade J, Schimmel P
Nat Commun 11 969 (2020)
PMID: 32080176

Function and Biology Details

Reaction catalysed: 
ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177340 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alanine--tRNA ligase, mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 113 amino acids
Theoretical weight: 11.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5JTZ9 (Residues: 873-985; Coverage: 12%)
Gene names: AARS2, AARSL, KIAA1270

Ligands and Environments

1 bound ligand:
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21
Unit cell:
a: 55.19Å b: 66.95Å c: 56.48Å
α: 90° β: 116.42° γ: 90°
R-values:
R R work R free
0.159 0.158 0.172
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

The molecular pathology of pathogenic mitochondrial tRNA variants.
Richter et al. (2021)
3 more