6nm9

Electron Microscopy
3.38Å resolution

CryoEM structure of the LbCas12a-crRNA-AcrVA4 dimer

Released:
Primary publication:
Structural Basis for the Inhibition of CRISPR-Cas12a by Anti-CRISPR Proteins.
Cell Host Microbe 25 815-826.e4 (2019)
PMID: 31155345
Related structures: EMD-9398

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-102728 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
AcrVA4 Chains: A, C
Molecule details ›
Chains: A, C
Length: 234 amino acids
Theoretical weight: 27.37 KDa
Source organism: Moraxella bovoculi
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0U2APF4 (Residues: 1-234; Coverage: 100%)
Gene name: AAX07_09545
Cpf1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 1227 amino acids
Theoretical weight: 143.75 KDa
Source organism: Lachnospiraceae bacterium ND2006
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A182DWE3 (Residues: 3-1228; Coverage: 100%)
Sequence domains:
RNA (25-MER) Chains: E, G
Molecule details ›
Chains: E, G
Length: 40 nucleotides
Theoretical weight: 12.88 KDa

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.38Å
Relevant EMDB volumes: EMD-9398
Expression system: Escherichia coli