6nmc

Electron Microscopy
4.24Å resolution

CryoEM structure of the LbCas12a-crRNA-2xAcrVA1 complex

Released:
Primary publication:
Structural Basis for the Inhibition of CRISPR-Cas12a by Anti-CRISPR Proteins.
Cell Host Microbe 25 815-826.e4 (2019)
PMID: 31155345
Related structures: EMD-0446

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-103171 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
Cpf1 Chain: A
Molecule details ›
Chain: A
Length: 1227 amino acids
Theoretical weight: 143.75 KDa
Source organism: Lachnospiraceae bacterium ND2006
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A182DWE3 (Residues: 3-1228; Coverage: 100%)
Sequence domains:
AcrVA1 Chains: B, C
Molecule details ›
Chains: B, C
Length: 165 amino acids
Theoretical weight: 19.65 KDa
Source organism: Moraxella bovoculi
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A5H1ZR46 (Residues: 1-165; Coverage: 100%)
Gene name: AAX09_07405
RNA Chain: G
Molecule details ›
Chain: G
Length: 40 nucleotides
Theoretical weight: 12.88 KDa

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.24Å
Relevant EMDB volumes: EMD-0446
Expression systems:
  • Escherichia coli
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'