6nme

Electron Microscopy
5.67Å resolution

Structure of LbCas12a-crRNA

Released:
Primary publication:
Structural Basis for the Inhibition of CRISPR-Cas12a by Anti-CRISPR Proteins.
Cell Host Microbe 25 815-826.e4 (2019)
PMID: 31155345
Related structures: EMD-0449

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103178 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Cpf1 Chain: A
Molecule details ›
Chain: A
Length: 1227 amino acids
Theoretical weight: 143.75 KDa
Source organism: Lachnospiraceae bacterium ND2006
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A182DWE3 (Residues: 3-1228; Coverage: 100%)
Sequence domains:
crRNA Chain: G
Molecule details ›
Chain: G
Length: 40 nucleotides
Theoretical weight: 12.88 KDa
Source organism: Lachnospiraceae bacterium ND2006
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 5.67Å
Relevant EMDB volumes: EMD-0449
Expression systems:
  • Escherichia coli
  • Not provided