› 6nmi

Electron Microscopy
3.7Å resolution

Cryo-EM structure of the human TFIIH core complex

Released:
DOI: 10.2210/pdb6nmi/pdb
PDB entry 6nmi coloured by chain, front view.
PDB entry 6nmi coloured by chain, side view.
PDB entry 6nmi coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The complete structure of the human TFIIH core complex.
Greber BJ, Toso DB, Fang J, Nogales E
Elife 8 (2019)
PMID: 30860024
Related structures: EMD-0452

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-148278 (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
General transcription and DNA repair factor IIH helicase/translocase subunit XPB Chain: A
Molecule details ›
Chain: A
Length: 653 amino acids
Theoretical weight: 74.23 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P19447 (Residues: 34-730; Coverage: 84%)
Gene names: ERCC3, XPB, XPBC
Sequence domains:
General transcription and DNA repair factor IIH helicase subunit XPD Chain: B
Molecule details ›
Chain: B
Length: 760 amino acids
Theoretical weight: 86.42 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P18074 (Residues: 1-760; Coverage: 100%)
Gene names: ERCC2, XPD, XPDC
Sequence domains:
General transcription factor IIH subunit 1 Chain: C
Molecule details ›
Chain: C
Length: 548 amino acids
Theoretical weight: 57.79 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P32780 (Residues: 1-539; Coverage: 98%)
Gene names: BTF2, GTF2H1
Sequence domains:
General transcription factor IIH subunit 4 Chain: D
Molecule details ›
Chain: D
Length: 462 amino acids
Theoretical weight: 52.25 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q92759 (Residues: 1-462; Coverage: 100%)
Gene name: GTF2H4
Sequence domains:
General transcription factor IIH subunit 2 Chain: E
Molecule details ›
Chain: E
Length: 366 amino acids
Theoretical weight: 39.61 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q13888 (Residues: 51-387; Coverage: 84%)
Gene names: BTF2P44, GTF2H2
Sequence domains:
General transcription factor IIH subunit 3 Chain: F
Molecule details ›
Chain: F
Length: 308 amino acids
Theoretical weight: 34.42 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q13889 (Residues: 1-308; Coverage: 100%)
Gene name: GTF2H3
Sequence domains: Transcription factor Tfb4
General transcription factor IIH subunit 5 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 8.06 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q6ZYL4 (Residues: 1-71; Coverage: 100%)
Gene names: C6orf175, GTF2H5, TTDA
Sequence domains: Transcription factor TFIIH complex subunit Tfb5
Structure domains: TFB5-like
CDK-activating kinase assembly factor MAT1 Chain: H
Molecule details ›
Chain: H
Length: 309 amino acids
Theoretical weight: 35.87 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P51948 (Residues: 1-309; Coverage: 100%)
Gene names: CAP35, MAT1, MNAT1, RNF66
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand SF4 1 x SF4
Ligand ZN 6 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-0452

Quick links

Citations

17 review citations

Envisioning how the prototypic molecular machine TFIIH functions in transcription initiation and DNA repair.
Tsutakawa et al. (2020)
16 more

23 mentions without citation

Structure and mechanism of the RNA polymerase II transcription machinery.
Schier et al. (2020)
22 more