› 6now

X-ray diffraction
4.1Å resolution

Human Mitochondrial Alanyl-tRNA Synthetase C-Ala domain

Released:
DOI: 10.2210/pdb6now/pdb
PDB entry 6now coloured by chain, front view.
PDB entry 6now coloured by chain, side view.
PDB entry 6now coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Relaxed sequence constraints favor mutational freedom in idiosyncratic metazoan mitochondrial tRNAs.
Kuhle B, Chihade J, Schimmel P
Nat Commun 11 969 (2020)
PMID: 32080176

Function and Biology Details

Reaction catalysed: 
ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177340 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alanine--tRNA ligase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 204 amino acids
Theoretical weight: 22.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5JTZ9 (Residues: 783-985; Coverage: 21%)
Gene names: AARS2, AARSL, KIAA1270

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: I41
Unit cell:
a: 104.42Å b: 104.42Å c: 164.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.233 0.282
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

The molecular pathology of pathogenic mitochondrial tRNA variants.
Richter et al. (2021)
3 more