› 6npy

Electron Microscopy
3.8Å resolution

Cryo-EM structure of NLRP3 bound to NEK7

Released:
DOI: 10.2210/pdb6npy/pdb
PDB entry 6npy coloured by chain, front view.
PDB entry 6npy coloured by chain, side view.
PDB entry 6npy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural mechanism for NEK7-licensed activation of NLRP3 inflammasome.
Sharif H, Wang L, Wang WL, Magupalli VG, Andreeva L, Qiao Q, Hauenstein AV, Wu Z, Núñez G, Mao Y, Wu H
Nature 570 338-343 (2019)
PMID: 31189953
Related structures: EMD-0476

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186218 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NACHT, LRR and PYD domains-containing protein 3 Chain: A
Molecule details ›
Chain: A
Length: 1034 amino acids
Theoretical weight: 117.89 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q96P20 (Residues: 3-1036; Coverage: 100%)
Gene names: C1orf7, CIAS1, NALP3, NLRP3, PYPAF1
Sequence domains:
Serine/threonine-protein kinase Nek7 Chain: B
Molecule details ›
Chain: B
Length: 279 amino acids
Theoretical weight: 32.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TDX7 (Residues: 33-302; Coverage: 89%)
Gene name: NEK7
Sequence domains: Protein kinase domain

Ligands and Environments

1 bound ligand:
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-0476
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

137 review citations

Inflammasome activation and regulation: toward a better understanding of complex mechanisms.
Zheng et al. (2020)
136 more

57 mentions without citation

Structure, Activation and Regulation of NLRP3 and AIM2 Inflammasomes.
Sharma et al. (2021)
56 more