6nq3

X-ray diffraction
2.89Å resolution

Crystal Structure of a SUZ12-RBBP4-PHF19-JARID2 Heterotetrameric Complex

Released:
Source organism: Homo sapiens
Primary publication:
A Dimeric Structural Scaffold for PRC2-PCL Targeting to CpG Island Chromatin.
Mol Cell 77 1265-1278.e7 (2020)
PMID: 31959557

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-170753 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Histone-binding protein RBBP4 Chains: A, E
Molecule details ›
Chains: A, E
Length: 439 amino acids
Theoretical weight: 49.37 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q09028 (Residues: 1-425; Coverage: 100%)
Gene names: RBAP48, RBBP4
Sequence domains:
Polycomb protein SUZ12 Chains: B, F
Molecule details ›
Chains: B, F
Length: 478 amino acids
Theoretical weight: 55.69 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q15022 (Residues: 76-545; Coverage: 64%)
Gene names: CHET9, JJAZ1, KIAA0160, SUZ12
PHD finger protein 19 Chains: C, G
Molecule details ›
Chains: C, G
Length: 84 amino acids
Theoretical weight: 9.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5T6S3 (Residues: 500-580; Coverage: 14%)
Gene names: PCL3, PHF19
Sequence domains: Polycomb-like MTF2 factor 2
Protein Jumonji Chains: D, H
Molecule details ›
Chains: D, H
Length: 19 amino acids
Theoretical weight: 2.26 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q92833 (Residues: 147-165; Coverage: 2%)
Gene names: JARID2, JMJ

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 127.71Å b: 139.6Å c: 268.08Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.17 0.23
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli
  • Not provided