› 6nqb

Electron Microscopy
3.8Å resolution

Role of Era in Assembly and Homeostasis of the Ribosomal Small Subunit

Released:
DOI: 10.2210/pdb6nqb/pdb
PDB entry 6nqb coloured by chain, front view.
PDB entry 6nqb coloured by chain, side view.
PDB entry 6nqb coloured by chain, top view.
Source organisms:
Primary publication:
Role of Era in assembly and homeostasis of the ribosomal small subunit.
Razi A, Davis JH, Hao Y, Jahagirdar D, Thurlow B, Basu K, Jain N, Gomez-Blanco J, Britton RA, Vargas J, Guarné A, Woodson SA, Williamson JR, Ortega J
Nucleic Acids Res 47 8301-8317 (2019)
PMID: 31265110
Related structures: EMD-0482

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
PDBe Complex ID:
PDB-CPX-112897 (preferred)
Entry contents:
15 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (16 distinct):
Small ribosomal subunit protein uS3 Chain: C
Molecule details ›
Chain: C
Length: 206 amino acids
Theoretical weight: 23.08 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7V3 (Residues: 2-207; Coverage: 88%)
Gene names: JW3276, b3314, rpsC
Sequence domains:
Small ribosomal subunit protein uS10 Chain: J
Molecule details ›
Chain: J
Length: 98 amino acids
Theoretical weight: 11.2 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7R5 (Residues: 5-102; Coverage: 95%)
Gene names: JW3283, b3321, nusE, rpsJ
Sequence domains: Ribosomal protein S10p/S20e
Small ribosomal subunit protein uS14 Chain: N
Molecule details ›
Chain: N
Length: 99 amino acids
Theoretical weight: 11.36 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AG59 (Residues: 2-101; Coverage: 98%)
Gene names: JW3269, b3307, rpsN
Sequence domains: Ribosomal protein S14p/S29e
30S ribosomal protein S19 Chain: S
Molecule details ›
Chain: S
Length: 74 amino acids
Theoretical weight: 8.44 KDa
Source organism: Escherichia coli H736
Expression system: Escherichia coli
Small ribosomal subunit protein uS4 Chain: D
Molecule details ›
Chain: D
Length: 205 amino acids
Theoretical weight: 23.38 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7V8 (Residues: 2-206; Coverage: 100%)
Gene names: JW3258, b3296, ramA, rpsD
Sequence domains:
Small ribosomal subunit protein uS5 Chain: E
Molecule details ›
Chain: E
Length: 149 amino acids
Theoretical weight: 15.68 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7W1 (Residues: 10-158; Coverage: 89%)
Gene names: JW3265, b3303, rpsE, spc
Sequence domains:
Small ribosomal subunit protein bS6, non-modified isoform Chain: F
Molecule details ›
Chain: F
Length: 93 amino acids
Theoretical weight: 10.97 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P02358 (Residues: 1-93; Coverage: 69%)
Gene names: JW4158, b4200, rpsF
Sequence domains: Ribosomal protein S6
Small ribosomal subunit protein uS8 Chain: H
Molecule details ›
Chain: H
Length: 129 amino acids
Theoretical weight: 14.02 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D8A1L7 (Residues: 2-130; Coverage: 99%)
Gene names: HMPREF9530_00381, rpsH
Sequence domains: Ribosomal protein S8
30S ribosomal protein S12 Chain: L
Molecule details ›
Chain: L
Length: 123 amino acids
Theoretical weight: 13.64 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Small ribosomal subunit protein uS15 Chain: O
Molecule details ›
Chain: O
Length: 86 amino acids
Theoretical weight: 9.94 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ADZ4 (Residues: 3-88; Coverage: 97%)
Gene names: JW3134, b3165, rpsO, secC
Sequence domains: Ribosomal protein S15
Small ribosomal subunit protein bS16 Chain: P
Molecule details ›
Chain: P
Length: 79 amino acids
Theoretical weight: 8.94 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7T3 (Residues: 1-79; Coverage: 96%)
Gene names: JW2590, b2609, rpsP
Sequence domains: Ribosomal protein S16
Small ribosomal subunit protein uS17 Chain: Q
Molecule details ›
Chain: Q
Length: 79 amino acids
Theoretical weight: 9.16 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AG63 (Residues: 4-82; Coverage: 94%)
Gene names: JW3273, b3311, neaA, rpsQ
Sequence domains: Ribosomal protein S17
30S ribosomal protein S18 Chain: R
Molecule details ›
Chain: R
Length: 52 amino acids
Theoretical weight: 6.11 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Small ribosomal subunit protein bS20 Chain: T
Molecule details ›
Chain: T
Length: 85 amino acids
Theoretical weight: 9.51 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7U7 (Residues: 3-87; Coverage: 98%)
Gene names: JW0022, b0023, rpsT
Sequence domains: Ribosomal protein S20
Small ribosomal subunit protein uS2 Chain: B
Molecule details ›
Chain: B
Length: 217 amino acids
Theoretical weight: 24.12 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7V0 (Residues: 10-226; Coverage: 90%)
Gene names: JW0164, b0169, rpsB
Sequence domains: Ribosomal protein S2
16S ribosomal RNA Chain: A
Molecule details ›
Chain: A
Length: 1542 nucleotides
Theoretical weight: 499.69 KDa
Sequence domains: Bacterial small subunit ribosomal RNA

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-0482
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The Impact of the Stringent Response on TRAFAC GTPases and Prokaryotic Ribosome Assembly.
Bennison et al. (2019)
5 more

1 mention without citation

Role of Era in assembly and homeostasis of the ribosomal small subunit.
Razi et al. (2019)