› 6nsb

X-ray diffraction
1.75Å resolution

Crystal structure of the IVR-165 (H3N2) influenza virus hemagglutinin in complex with 6'-SLNLN

Released:
DOI: 10.2210/pdb6nsb/pdb
PDB entry 6nsb coloured by chain, front view.
PDB entry 6nsb coloured by chain, side view.
PDB entry 6nsb coloured by chain, top view.
Source organism: Influenza A virus (A/Victoria/361/2011(H3N2))
Primary publication:
Preventing an Antigenically Disruptive Mutation in Egg-Based H3N2 Seasonal Influenza Vaccines by Mutational Incompatibility.
Wu NC, Lv H, Thompson AJ, Wu DC, Ng WWS, Kadam RU, Lin CW, Nycholat CM, McBride R, Liang W, Paulson JC, Mok CKP, Wilson IA
Cell Host Microbe 25 836-844.e5 (2019)
PMID: 31151913

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125747 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (5 distinct):
Hemagglutinin Chain: A
Molecule details ›
Chain: A
Length: 321 amino acids
Theoretical weight: 35.86 KDa
Source organism: Influenza A virus (A/Victoria/361/2011(H3N2))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: L0HR89 (Residues: 27-345; Coverage: 56%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin (Ha1 Chain); Chain: A; domain 1
Hemagglutinin Chain: B
Molecule details ›
Chain: B
Length: 176 amino acids
Theoretical weight: 20.15 KDa
Source organism: Influenza A virus (A/Victoria/361/2011(H3N2))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: L0HR89 (Residues: 346-521; Coverage: 31%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin Ectodomain; Chain B

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, GAL, SIA
Carbohydrate polymer
1 bound ligand:
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: R32
Unit cell:
a: 101.123Å b: 101.123Å c: 387.229Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.166 0.165 0.187
Expression system: Trichoplusia ni

Quick links

Citations

11 review citations

Emerging Concepts and Technologies in Vaccine Development.
Brisse et al. (2020)
10 more

1 mention without citation

Preventing an Antigenically Disruptive Mutation in Egg-Based H3N2 Seasonal Influenza Vaccines by Mutational Incompatibility.
Wu et al. (2019)