Function and Biology Details
Reactions catalysed:
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
GTP + a 5'-diphospho-[mRNA] = diphosphate + a 5'-(5'-triphosphoguanosine)-[mRNA]
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
- Non-structural protein NSP8 superfamily, coronavirus
- Non-structural protein NSP8, coronavirus
- Non-structural protein NSP7 superfamily, coronavirus
- Non-structural protein NSP7, coronavirus
- RNA-directed RNA polymerase, catalytic domain
- DNA/RNA polymerase superfamily
- RNA-directed RNA polymerase, C-terminal domain
- RNA-dependent RNA polymerase, coronavirus
3 more domains
Structure analysis Details
Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143078 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
RNA-directed RNA polymerase nsp12
Molecule details ›
Chain: A
Length: 955 amino acids
Theoretical weight: 109.28 KDa
Source organism: Severe acute respiratory syndrome-related coronavirus
Expression system: Spodoptera frugiperda
UniProt:
Sequence domains:
Length: 955 amino acids
Theoretical weight: 109.28 KDa
Source organism: Severe acute respiratory syndrome-related coronavirus
Expression system: Spodoptera frugiperda
UniProt:
- Canonical:
P0C6X7 (Residues: 4370-5300; Coverage: 13%)
Sequence domains:
Non-structural protein 8
Molecule details ›
Chains: B, D
Length: 198 amino acids
Theoretical weight: 21.89 KDa
Source organism: Severe acute respiratory syndrome-related coronavirus
Expression system: Escherichia coli BL21
UniProt:
Sequence domains: Coronavirus replicase NSP8
Length: 198 amino acids
Theoretical weight: 21.89 KDa
Source organism: Severe acute respiratory syndrome-related coronavirus
Expression system: Escherichia coli BL21
UniProt:
- Canonical: P0C6U8 (Residues: 3920-4117; Coverage: 5%)
Sequence domains: Coronavirus replicase NSP8
Non-structural protein 7
Molecule details ›
Chain: C
Length: 84 amino acids
Theoretical weight: 9.33 KDa
Source organism: Severe acute respiratory syndrome-related coronavirus
Expression system: Escherichia coli BL21
UniProt:
Sequence domains: Coronavirus replicase NSP7
Length: 84 amino acids
Theoretical weight: 9.33 KDa
Source organism: Severe acute respiratory syndrome-related coronavirus
Expression system: Escherichia coli BL21
UniProt:
- Canonical: P0C6X7 (Residues: 3837-3919; Coverage: 1%)
Sequence domains: Coronavirus replicase NSP7
