› 6nv1

X-ray diffraction
2.5Å resolution

Structure of drug-resistant V27A mutant of the influenza M2 proton channel bound to spiroadamantyl amine inhibitor

Released:
DOI: 10.2210/pdb6nv1/pdb
Model geometry
Fit model/data
PDB entry 6nv1 coloured by chain, front view.
PDB entry 6nv1 coloured by chain, side view.
PDB entry 6nv1 coloured by chain, top view.
Source organism: Influenza A virus (A/Indonesia/CDC1031RE2/2007(H5N1))
Primary publication:
X-ray Crystal Structures of the Influenza M2 Proton Channel Drug-Resistant V27A Mutant Bound to a Spiro-Adamantyl Amine Inhibitor Reveal the Mechanism of Adamantane Resistance.
Thomaston JL, Konstantinidi A, Liu L, Lambrinidis G, Tan J, Caffrey M, Wang J, Degrado WF, Kolocouris A
Biochemistry 59 627-634 (2020)
PMID: 31894969

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107264 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Matrix protein 2 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 27 amino acids
Theoretical weight: 2.73 KDa
Source organism: Influenza A virus (A/Indonesia/CDC1031RE2/2007(H5N1))
Expression system: Not provided
UniProt:
  • Canonical: A4D7H3 (Residues: 22-46; Coverage: 26%)
Gene names: M, M2

Ligands and Environments

3 bound ligands:
Ligand OLC 8 x OLC
Ligand E01 2 x E01
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 49.806Å b: 49.95Å c: 75.029Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.22 0.258
Expression system: Not provided

Quick links

Citations

2 review citations

Antiviral Approaches against Influenza Virus.
Kumari et al. (2023)
1 more

4 mentions without citation

Put a cork in it: Plugging the M2 viral ion channel to sink influenza.
Jalily et al. (2020)
3 more