› 6nwa

Electron Microscopy
3.48Å resolution

The structure of the photosystem I IsiA super-complex

Released:
DOI: 10.2210/pdb6nwa/pdb
PDB entry 6nwa coloured by chain, front view.
PDB entry 6nwa coloured by chain, side view.
PDB entry 6nwa coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803
Primary publication:
The structure of the stress-induced photosystem I-IsiA antenna supercomplex.
Toporik H, Li J, Williams D, Chiu PL, Mazor Y
Nat Struct Mol Biol 26 443-449 (2019)
PMID: 31133699
Related structures: EMD-0524

Function and Biology Details

Reaction catalysed: 
Reduced plastocyanin + oxidized ferredoxin + light = oxidized plastocyanin + reduced ferredoxin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 51-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146414 (preferred)
Entry contents:
12 distinct polypeptide molecules
Macromolecules (12 distinct):
Photosystem I P700 chlorophyll a apoprotein A1 Chains: A, H, a
Molecule details ›
Chains: A, H, a
Length: 751 amino acids
Theoretical weight: 83.02 KDa
Source organism: Synechocystis sp. PCC 6803
UniProt:
  • Canonical: P29254 (Residues: 1-751; Coverage: 100%)
Gene names: psaA, slr1834
Sequence domains: Photosystem I psaA/psaB protein
Photosystem I P700 chlorophyll a apoprotein A2 Chains: B, G, b
Molecule details ›
Chains: B, G, b
Length: 731 amino acids
Theoretical weight: 81.37 KDa
Source organism: Synechocystis sp. PCC 6803
UniProt:
  • Canonical: P29255 (Residues: 1-731; Coverage: 100%)
Gene names: psaB, slr1835
Sequence domains: Photosystem I psaA/psaB protein
Photosystem I iron-sulfur center Chains: C, N, c
Molecule details ›
Chains: C, N, c
Length: 81 amino acids
Theoretical weight: 8.84 KDa
Source organism: Synechocystis sp. PCC 6803
UniProt:
  • Canonical: P32422 (Residues: 1-81; Coverage: 100%)
Gene names: psaC, ssl0563
Sequence domains: 4Fe-4S dicluster domain
Photosystem I reaction center subunit II Chains: D, O, d
Molecule details ›
Chains: D, O, d
Length: 141 amino acids
Theoretical weight: 15.66 KDa
Source organism: Synechocystis sp. PCC 6803
UniProt:
  • Canonical: P19569 (Residues: 1-141; Coverage: 100%)
Gene names: psaD, slr0737
Sequence domains: PsaD
Photosystem I reaction center subunit IV Chains: E, P, e
Molecule details ›
Chains: E, P, e
Length: 74 amino acids
Theoretical weight: 8.15 KDa
Source organism: Synechocystis sp. PCC 6803
UniProt:
  • Canonical: P12975 (Residues: 1-74; Coverage: 100%)
Gene names: psaE, ssr2831
Sequence domains: Photosystem I reaction centre subunit IV / PsaE
Photosystem I reaction center subunit III Chains: F, Q, f
Molecule details ›
Chains: F, Q, f
Length: 165 amino acids
Theoretical weight: 18.27 KDa
Source organism: Synechocystis sp. PCC 6803
UniProt:
  • Canonical: P29256 (Residues: 1-165; Coverage: 100%)
Gene names: psaF, sll0819
Sequence domains: Photosystem I reaction centre subunit III
Photosystem I reaction center subunit VIII Chains: I, R, i
Molecule details ›
Chains: I, R, i
Length: 40 amino acids
Theoretical weight: 4.41 KDa
Source organism: Synechocystis sp. PCC 6803
UniProt:
  • Canonical: Q55330 (Residues: 1-40; Coverage: 100%)
Gene names: psaI, smr0004
Sequence domains: Photosystem I reaction centre subunit VIII
Photosystem I reaction center subunit IX Chains: J, S, j
Molecule details ›
Chains: J, S, j
Length: 40 amino acids
Theoretical weight: 4.54 KDa
Source organism: Synechocystis sp. PCC 6803
UniProt:
  • Canonical: Q55329 (Residues: 1-40; Coverage: 100%)
Gene names: psaJ, sml0008
Sequence domains: Photosystem I reaction centre subunit IX / PsaJ
Photosystem I reaction center subunit XI Chains: L, U, l
Molecule details ›
Chains: L, U, l
Length: 157 amino acids
Theoretical weight: 16.63 KDa
Source organism: Synechocystis sp. PCC 6803
UniProt:
  • Canonical: P37277 (Residues: 1-157; Coverage: 100%)
Gene names: psaL, slr1655
Sequence domains: Photosystem I reaction centre subunit XI
Photosystem I reaction center subunit XII Chains: M, V, m
Molecule details ›
Chains: M, V, m
Length: 31 amino acids
Theoretical weight: 3.38 KDa
Source organism: Synechocystis sp. PCC 6803
UniProt:
  • Canonical: P72986 (Residues: 1-31; Coverage: 100%)
Gene names: psaM, smr0005
Sequence domains: Photosystem I protein M (PsaM)
Iron stress-induced chlorophyll-binding protein Chains: W, X, Y, Z, g, h, n, o, p, q, r, s, t, u, v, w, x, y
Molecule details ›
Chains: W, X, Y, Z, g, h, n, o, p, q, r, s, t, u, v, w, x, y
Length: 342 amino acids
Theoretical weight: 37.25 KDa
Source organism: Synechocystis sp. PCC 6803
UniProt:
  • Canonical: Q55274 (Residues: 1-342; Coverage: 100%)
Gene names: isiA, sll0247
Sequence domains: Photosystem II protein
Photosystem I reaction center subunit PsaK 1 Chains: K, T, k
Molecule details ›
Chains: K, T, k
Length: 86 amino acids
Theoretical weight: 8.65 KDa
Source organism: Synechocystis sp. PCC 6803
UniProt:
  • Canonical: P72712 (Residues: 1-86; Coverage: 100%)
Gene names: psaK, psaK1, ssr0390
Sequence domains: Photosystem I psaG / psaK

Ligands and Environments

9 bound ligands:
Ligand LHG 9 x LHG
Ligand LMG 15 x LMG
Ligand CL0 3 x CL0
Ligand CLA 588 x CLA
Ligand PQN 6 x PQN
Ligand SF4 9 x SF4
Ligand BCR 135 x BCR
Ligand LMT 3 x LMT
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.48Å
Relevant EMDB volumes: EMD-0524

Quick links

Citations

3 review citations

Opportunities and challenges for assigning cofactors in cryo-EM density maps of chlorophyll-containing proteins.
Gisriel et al. (2020)
2 more

4 mentions without citation

A Novel Mode of Photoprotection Mediated by a Cysteine Residue in the Chlorophyll Protein IsiA.
Chen et al. (2021)
3 more